Amplificación Isotérmica de Ácidos Nucleicos
El futuro del diagnóstico molecular
DOI:
https://doi.org/10.29105/bys9.17-235Keywords:
Molecular diagnosis, qPCR, DNA, RNA, NASBA, LAMP, RPAAbstract
Molecular diagnostics is a key tool with applications in human health, veterinary science, and food safety, among other areas. In human health, the COVID-19 pandemic spurred the widespread use of real time polymerase chain reaction (also known as qPCR when it
when used in quantitative analysis) tests, enabling large-scale screenings and millions of diagnoses worldwide, establishing qPCR as the most widely used molecular technique in history. However, access to these tests was not equitable across countries, as qPCR requires specialized infrastructure and costly equipment. To address this limitation, nucleic acid isothermal amplification has emerged as a revolutionary alternative in molecular diagnostics, enabling rapid and accurate detection of pathogens and biomarkers without the need for sophisticated equipment. This article discusses three methods shaping the future of molecular detection: NASBA (Nucleic Acid Sequence-Based Amplification), LAMP (Loop-Mediated Isothermal Amplification), and RPA (Recombinase Polymerase Amplification). Each technique offers advantages in sensitivity, specificity, and ease of use, making them particularly suitable for low-resource settings and point-of-care diagnostics. These technologies not only have the potential to revolutionize diagnostics but also to enable a rapid and effective response to future health crises.
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Copyright (c) 2026 Everardo González-González, Margarita de la Luz Martínez-Fierro, Idalia Garza-Veloz, Gerardo de Jesús Trujillo-Rodríguez, Iván Delgado-Enciso, Elda A. Flores-Contreras, Iram Pablo Rodríguez-Sánchez
This work is licensed under a Creative Commons Attribution 4.0 International License.
